Simultaneous Quantitation of Boric Acid and Calcium Fructoborate in Dietary Supplements by HPTLC–Densitometry

Abstract

The paper describes a new, simple, selective and precise high-performance thin-layer chromatographic (HPTLC) method for the simultaneous identification and quantitative determination of boric acid (BA) and calcium fructoborate (CFB) in bulk and tablet/capsule dosage forms of dietary supplements. HPTLC silica gel G 60 F₂₅₄ precoated glass plates were used as the stationary phase. The mobile phase consisted of 2-propanol–water 8:2 (v/v). The two boron-based compounds were adequately separated with the R_f values of 0.83 ± 0.01 (BA) and 0.59 ± 0.01 (CFB).