Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
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Determination of Menadione by Liquid Chromatography–Tandem Mass Spectrometry Using Pseudo Multiple Reaction Monitoring
Maya KAMAOYoshihisa HIROTAYoshitomo SUHARANaoko TSUGAWAKimie NAKAGAWAToshio OKANOHiroshi HASEGAWA
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Supplementary material

2017 Volume 33 Issue 7 Pages 863-867

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Abstract

This study aimed to develop a menadione (MD) determination method employing liquid chromatography–tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 – 8.2%, with the corresponding recoveries equaling 90.5 – 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

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© 2017 by The Japan Society for Analytical Chemistry
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