Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Volume 33 , Issue 7
Showing 1-20 articles out of 20 articles from the selected issue
Highlights
Original Papers
  • Meiling CHEN, Jiezhao ZHOU, Le MEI, Fanglin YU, Xiangyang XIE, Yan LIU ...
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 755-759
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    In order to overcome deficiencies for simultaneously determining felodipine (FDP) and metoprolol (MPL) with low recovery and low sensitivity, a new online SPE coupled with the liquid chromatography–tandem mass spectrometry (SPE-LC-MS/MS) method for the simultaneous quantitative determination of FDP and MPL in beagle dog plasma was established. The SPE extraction of FDP and MPL was performed on a Retain PEP Javelin column (10 × 2.1 mm, 5 μm), while the chromatographic separation was achieved on a ZORBAX SB-C18 (50 × 2.1 mm, 3.5 μm) analytical column. Multiple reaction monitoring operated in the positive ion mode was adopted in MS detection, and the precursors to the product ion transition values of m/z 384/338.1, 268/74.2 and 436.2/207.1 were used to measure FDP, MPL and the internal standard (valsartan). The high throughput, accurate and sensitive method for FDP and MPL was validated and applied to the bioavailability research of FDP and MPL in beagle dogs.
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  • Xiaohan SUN, Shenghong YANG, Mingzhen GUO, Shuang MA, Mingda ZHENG, Ji ...
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 761-767
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    An “on-off-on” mode was developed for the detection of mercury ion (Hg2+) and glutathione (GSH) with high sensitivity and selectivity based on the nitrogen-doped carbon dots (N-CDs) fluorescent probe. The N-CDs were synthesized through microwave treatment of citric acid and diethylenetriamine for 2 min, and exhibited excellent fluorescence properties and high quantum yield (27.7%). The fluorescence intensity of the N-CDs could be significantly quenched by Hg2+ (turn-off). Upon addition of GSH, the fluorescence intensity of the N-CDs-Hg2+ system could be recovered clearly (turn-on). The limit of detection of Hg2+ and GSH was 23 and 59 nM, respectively. Moreover, the “on-off-on” probe was successfully applied to the determination of Hg2+ in tap water and water from the Yellow River. Meanwhile, due to bright luminescence, good biocompatibility and low cytotoxicity, the N-CDs-based probe was successfully employed as visualizing the intracellular Hg2+ and GSH sensors in live HeLa cell.
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  • Leila DOLATYARI, Mohammad Reza YAFTIAN, Sadegh ROSTAMNIA, Mir Saeed SE ...
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 769-776
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    A solid-phase extraction based on a functionalized SBA-15, with the Schiff base ligand, ethylenediaminepropylesalicylaldimine (SBA/EnSA), was developed for the recovery and preconcentration of trace amounts of uranium(VI) in water samples, prior to its determination spectrophotometrically using ArsenazoIII. For optimizing the parameters affecting the adsorption step (pH, adsorbent dose and adsorption time) and those influencing the desorption process (concentration and volume of eluent and desorption time), a statistical technique response surface methodology (RSM) was employed. The limit of detection and the linear dynamic range for the proposed method were 10 μg L−1, and 33.5 – 500 μg L−1, respectively. The adsorbent showed a high capacity (110.2 mg g−1) and the method allowed obtaining a preconcentration factor of 67. The inter- and intra-day relative standard deviations for a solution of 100 μg L−1 (n = 5) were found to be 4.8 – 6.2%. The developed method was successfully applied for the determination of U(VI) in water samples.
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  • Heng WANG, Liangpo LIU, Syed Ali Musstjab Akber Shah EQANI, Heqing SHE ...
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 777-781
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    To quantitatively measure trace levels of bisphenol A (BPA) in infant urine, a simply improved high-performance liquid chromatography (HPLC)–tandem mass-spectrometry method was developed and validated. In the present work, a ZORBAX SB-C18 column (2.1 × 30 mm, 3.5 μm) was used for trapping and isolating BPA from the HPLC mobile phase. The result showed that the trapping column can provide effective separation from the background BPA to the sample BPA. This method has a limit of detection (LOD) of 0.048 μg/L, spiked recoveries 85.4% (0.50 – 10.0 μg/L), and relative standard deviations of 1.8 – 15.9%. By using its low-LOD advantage, we firstly reported that Chinese infants (n = 48 with age <6 months) have been ubiquitously exposed to BPA (detection frequency of 93%) with an median level of 0.13 μg/L (ranging from below LOD to 5.04 μg/L).
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  • Chao HU, Xiang Juan KONG, Ru Qin YU, Ting Ting CHEN, Xia CHU
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 783-788
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    A novel fluorescence sensing platform for ultrasensitive detection of S1 nuclease activity has been constructed based on MnO2 nanosheets and FAM labeled single-stranded DNA (FAM-ssDNA). In this system, MnO2 nanosheets were found to have different adsorbent ability toward ssDNA and mono- or oligonucleotide fragments. FAM-ssDNA could adsorb on MnO2 nanosheets and resulted in significant fluorescence quenching through fluorescence resonance energy transfer (FRET), while mono- or oligonucleotide fragments could not adsorb on MnO2 nanosheets and still retained strong fluorescence emission. With the addition of S1 nuclease, FAM-ssDNA was cleaved into mono- or oligonucleotide fragments, which were not able to adsorb on MnO2 nanosheets and the fluorescence signal was never quenched. The different fluorescence intensity allowed for examination of S1 nuclease activity. The developed method can detect S1 nuclease activity in the range of 0 – 20 U mL−1 with a detection limit of 0.05 U mL−1. Benefits of the system include less time-consuming processes and more simple design compared to other endonuclease assays. Satisfactory performance for S1 nuclease in complex samples has been successfully demonstrated with the system. The developed assay could potentially provide a new platform in bioimaging and clinical diagnosis.
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  • Si-qin-gao-wa HAN, Lin BAO, Mei-Ling ZHANG, Xiang LIN, Wu-Li-Ji HASI
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 789-792
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    Diazepam injection was detected based on a droplet surface enhanced Raman spectroscopy (SERS) platform, which overcomes the disadvantages of the poor uniformity and time-consuming sample treatment process of the conventional “drop and dry” detection strategy. The Raman peak positions of diazepam injection were determined and identified, they are mainly located in the 689, 1002, 1170 and 1598 cm−1, etc. Different concentrations of diazepam injection were detected. It was found that the intensity of 1002 cm−1 increases linearly with concentration in the range from 0.05 to 10 μg/mL and the linear correlation coefficient is 0.988. The limit of detection can reach 0.05 μg/mL. The SERS method is easy, fast and efficient. The results are accurate and reliable. It has favorable application potential in the detection of diazepam injection.
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  • Yan WANG, Bin-Bin HUANG, Wan-Lin DAI, Bin XU, Tian-Liang WU, Jia-Ping ...
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 793-799
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    A sensitive capsaicin sensor was constructed based on a poly(sodium 4-styrenesulfonate) functionalized graphite modified screen printed electrode (PSS-Grp/SPE) in this study. The PSS-Grp and poly(diallyldimethylammonium chloride) functionalized graphite (PDDA-Grp) were easily synthesized by interacting Grp with PSS and PDDA through sonication, and resulted in negative and with positive charges on the surface, respectively. The prepared PSS-Grp and PDDA-Grp were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and ultraviolet and visible spectroscopy (UV-vis). The electrochemical performance of PSS-Grp in a 50 μM capsaicin solution presented a current density of 33 μA cm−2, which was much higher than the PDDA-Grp of 1.5 μA cm−2. Our study showed that capsaicin could interact better with strong negative charges on the PSS-Grp/SPE surface to give a higher electrochemical response. The direct electrochemical sensing of capsaicin was achieved at PSS-Grp/SPE using differential pulse stripping voltammetry (DPSV) under the optimized conditions.
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  • Trisna K. SARI, Jiye JIN, Rahmiana ZEIN, Edison MUNAF
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 801-806
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    A square-wave anodic stripping voltammetry (SWASV) for the determination of trace amounts of hexavalent chromium Cr(VI) at a graphite/styrene-acrylonitrile (Graphite-SAN) copolymer composite electrode is described. This method involves a preconcentration step whereby the trace Cr(VI) was cathodically reduced to Cr(III) on an electrode surface in an acetate buffer (pH 5), followed by an anodic stripping technique with a square-wave voltammetric mode. It has been shown that the analytical sensitivity is significantly improved at the Graphite-SAN copolymer composite electrode in comparison with the conventional glassy carbon electrode, due to the strong interaction between Cr(III) and the nitrile end group of the SAN copolymer. The SWASV response was characterized with respect to the pH, deposition potential, possible interferences, etc. Under the optimal conditions, the stripping peak height linearly increased with the concentration of Cr(VI) in a range from 0 to 150 ng mL−1 with a correlation coefficient of 0.997, and a detection limit of 4.2 ng mL−1 was achieved based on signal-to-noise ratio of about 3. The Graphite-SAN composite electrode exhibited some interesting advantages, such as high mechanical rigid, easy surface renewable, higher sensitivity and better peak resolution in comparison with the results at conventional glassy carbon electrodes. They have been applied to the determination of Cr(VI) in real water samples with satisfactory recoveries.
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  • Nil OZBEK, Gulcin Torunoglu TURAN, Bahire Filiz SENKAL, Suleyman AKMAN
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 807-811
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    In this study, at first the synthesis of 3-chloro-2-hydroxypropyl methacrylate-ethylene glycole dimethacrylate co-polymer beads and its modification with tris(2-aminoethyl) amine is described. Characterization of the polymer was done by FTIR and SEM. The functional co-polymer was filled in a disposable pipet tip and tightly connected to a 50-mL syringe for the separation and the enrichment of lead and cadmium prior to their determination by flame atomic absorption spectrometry. The sample and then the eluate were subsequently drawn and discharged to retain and desorb lead and cadmium by means of the syringe, respectively. Both analytes were quantitatively retained at pH 4 and eluted using 3.0 mol L−1 of HNO3 at flow rates of approximately 10 mL min−1. Under the optimum conditions, the enrichment factors of up to 50-fold both elements could be obtained by drawing and discharging 250 mL (5 × 50 mL) of the sample, and then 5 mL of the eluent. The recoveries were >90%. The limits of detection (3σ; N = 10 of blank) for Pb and Cd were 0.0034 and 0.0016 mg L−1 for a 50-fold enrichment, respectively. The analyte concentrations in a certified waste water reference agreed within the certified values in the 95% confidence range.
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  • Tomoya IWATA, Hirohisa NAGATANI, Toshiyuki OSAKAI
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 813-819
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    The fluorescence behaviors of potential-sensitive dyes including anionic DiBAC4(3) (denoted by dye A), DiSBAC2(3) (dye B), and zwitterionic di-4-ANEPPS (dye C) were studied in oil-in-water (O/W) emulsions. In this study, the equilibrium Galvani potential difference (ΔOWφeq) of the O/W-emulsion droplets was controlled by changing the ratio of the concentrations of electrolytes added to the O (=1,2-dichloroethane) and W phases. When using an adequate combination of the dyes, i.e., B and C, we could observe that the ratio of their fluorescence peak intensities was changed from 1.08 to 1.38, depending on the change of (ΔOWφeq from 26 to 73 mV. It is desirable to apply this method to study the potential-dependent ion or electron-transfer reactions occurring at vesicles or liposomes, and also to biomembranes.
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  • Young-Min KIM, Jae Woo KIM, Hye Mi MOON, Min-Jin LEE, Akihiko HOSAKA, ...
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 821-824
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    Analysis of a residual solvent in polymeric materials has become an important issue due to the increased regulations and standards for its use. N-Methyl-2-pyrrolidone (NMP) is a solvent widely used in many industries and restricted as one of the chemicals under EU REACH regulations due to its potential harmful effects. In this study, thermal desorption–gas chromatography/mass spectrometry (TD-GC/MS) is applied for the quantitative analysis of NMP with the use of a polymer-coated sample cup. By using the polymer-coated sample cup, the vaporization of NMP was prevented during waiting time before TD-GC/MS analysis. The calibration curve for the TD method showed good linearity (correlation coefficient, r2 = 0.9998) and precision values (below 5.3% RSD). NMP recovery rates in different polymer matrices (PS, PMMA and PVC) were in the range of 98.8 to 106.6% with RSD values below 5.0%. The quantification result (600 mg NMP/kg PVC) for the blind NMP carrying sample in a PVC matrix by TD-GC/MS was higher than that (532 mg NMP/kg PVC) by solvent extraction-GC/MS method.
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  • Hisashi SATOH, Yuji MIYAZAKI, Shou TANIUCHI, Mamoru OSHIKI, Rathnayake ...
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 825-830
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    An ionophore-doped sensing membrane phosphate (PO4) microsensor based on bis(dibromophenylstannyl)methane (Bis microsensor) is described. The Bis microsensor showed a Nernstian response. The response of the Bis microsensor was log-linear down to a monohydrogen phosphate ion (HPO42−) concentration of 0.5 μM (corresponding to 1.0 μM of orthophosphate at pH 7.2), whereas the detection limit of PO4-microsensors based on trialkyl/aryltin chloride was 50 μM of HPO42−. The Bis microsensor showed excellent selectivity for HPO42− against nitrite, nitrate, chloride, bicarbonate and sulfate, as compared with PO4 microsensors based on trialkyl/aryltin chloride. Dissolved oxygen, which is known to interfere with the response of a previously developed cobalt-based potentiometric solid-state PO4 microsensor, had no effect on the response of the ionophore-doped sensing membrane-type microsensors described herein. Only OH (i.e., pH) interfered with the ionophore-doped sensing membrane-type microsensors.
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  • M. Lutfi FIRDAUS, Ikka FITRIANI, Santhy WYANTUTI, Yeni W. HARTATI, Ren ...
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 831-837
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    Novel green-chemistry synthesis of silver nanoparticles (AgNPs) is introduced as a low-cost, rapid and easy-to-use analytical method for mercury ion detection. Aqueous fruit extract of water apple (Syzygium aqueum) was used for the first time as bioreductant to synthesize stable AgNPs. The prepared AgNPs have a yellowish-brown color with a surface plasmon resonance peak at 420 nm. The addition of Hg(II) ions then changes the AgNPs color to colorless. The color change was in proportion to the concentration of Hg(II) ions. The presence of other metal ions in the system was also evaluated. The proposed method shows good selectivity and sensitivity towards Hg(II) ions. Using UV-visible spectrophotometry, the detection limit of the developed method was 8.5 × 10−7 M. The proposed method has been successfully applied for determination of Hg(II) ions in tap and lake water samples with precision better than 5%.
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  • Hong-qi XIA, Yuki KITAZUMI, Osamu SHIRAI, Kenji KANO
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 839-844
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    Non-catalytic direct electron transfer (DET) signal of Compound I of horseradish peroxidase (POD) was first detected at 0.7 V on POD/carbon nanotube mixture-modified electrodes. Excellent performance of DET-type bioelectrocatalysis was achieved with POD immobilized with glutaraldehyde on Ketjen Black (KB)-modified electrodes for H2O2 reduction with an onset potential of 0.65 V (vs. Ag | AgCl | sat. KCl) without any electrode surface modification. The POD-immobilized KB electrode was found to be suitable for detecting H2O2 with a low detection limit (0.1 μM at S/N = 3) at –0.1 V. By co-immobilizing glucose oxidase (GOD) and POD on the KB-modified electrode, a bienzyme electrode was constructed to couple the oxidase reaction of GOD with the DET-type bioelectrocatalytic reduction of H2O2 by POD. The amperometric detection of glucose was performed with a high sensitivity (0.33 ± 0.01 μA cm−2 μM−1) and a low detection limit (2 μM at S/N = 3).
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  • Yukina MATSUI, Katsumi HAMAMOTO, Yuki KITAZUMI, Osamu SHIRAI, Kenji KA ...
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 845-851
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    We performed numerical simulations on an extremely fast, mediated, electron transfer-type bioelectrocatalytic reaction using a microband electrode. The simulations under fast-enzyme-kinetics conditions predicted that the decrement of the current density by increaseing the microband thickness would effectively improve the upper limit of detection. These predictions were accurate for an ultrathin-ring with thickness of 100 nm and gold leaf with thickness of 10 μm electrodes, acting as novel amperometric glucose sensors with FAD-dependent glucose dehydrogenase. The gold leaf electrode provided pseudo-steady-state currents which were proportional to the glucose concentration up to a concentration of 20 times higher than the mediator concentration.
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  • Kosuke HARA, Taka-aki YANO, Kota SUZUKI, Masaaki HIRAYAMA, Tomohiro HA ...
    Type: Original Papers
    2017 Volume 33 Issue 7 Pages 853-858
    Published: July 10, 2017
    Released: July 10, 2017
    JOURNALS FREE ACCESS
    Local crystalline structures of LiCoO2 nanothin film cathodes in a lithium ion battery have been spectroscopically elucidated through confocal Raman imaging analysis at high spatial resolution of several hundred nanometers. A significant difference in the crystalline structure is found between the nanometric thin films and bulk powders. Thermally induced local decomposition of LiCoO2 into an impurity phase on the films has also been revealed along with the mechanism of the temperature-triggered decomposition process. Moreover, frequency-based Raman imaging enables us to locally probe spatial separation between stoichiometric (LiCoO2) and non-stoichiometric (Li1-xCoO2, 0 < x < 1) crystal phases on the thin films. Such local crystalline analysis is a promising approach to provide new insights into the degradation mechanism of lithium-ion batteries, which would result in improving the performance of thin film-based lithium ion batteries.
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