Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Dynamic Measurement Method for Bio-molecular Interactions by Using Centrifugal Force
Mao OTAKEYoshiaki UKITA
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Supplementary material

2019 Volume 35 Issue 10 Pages 1123-1127

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Abstract

Measurement of single molecule interaction is performed by various micromanipulation techniques. However, a complex operation is required to perform the measurement, so the measurement throughput is low and a large amount of time is required for statistical analysis. In this paper, we propose a method for measuring intermolecular interactions that applies a tensile force to the intermolecular bond by using centrifugal force and can easily measure binding force. The binding force of an antigen–antibody reaction system was measured by the use of the proposed method as a proof of concept. The measured binding forces were distributed in the range of 19 – 133 pN. The values obtained by use of the proposed method were consistent with the conventional method. This result is promising for applications that require further development, such as the development of a biosensor as a novel measuring technique that is based on the intermolecular interaction measurement.

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© 2019 by The Japan Society for Analytical Chemistry
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