Development of a Sensitive Chemiluminescent Immunoassay for the Determination of 3-Methyl-quinoxaline-2-carboxylic Acid and Quinoxaline-2-carboxylic Acid in Edible Animal Tissues Using Immunomagnetic Beads Capturing

Abstract

We have reported a sensitive chemiluminescent competitive indirect enzyme-linked immunosorbent assay (CL-ciELISA) based on immunomagnetic beads separation, purification and enrichment for the simultaneous determination of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) and quinoxaline-2-carboxylic acid (QCA) in edible animal tissues. Forty field samples were analyzed with the developed CL-ciELISA, and the results correlated well with those obtained in liquid chromatography–tandem mass spectrometry (LC-MS/MS), confirming the utility of CL-ciELISA for the quantitation of MQCA and QCA in fish, shrimp, pork and chicken with a good accuracy.