A Solid-state Electrochemiluminescence Sensor for Detecting Glutathione with a Graphite-phase Carbon Nitride/Silica Modified Glassy Carbon Electrode

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A solid-state electrochemiluminescence (ECL) sensor for the detection of reduced glutathione (GSH) based on a g-C₃N₄/SiO₂ modified glass carbon electrode (GCE) has been developed in this research. The g-C₃N₄, which is employed as a luminophore, is simply prepared and exhibits an excellent ECL response. Mesoporous silica hollow spheres (SiO₂) with a large specific surface area are introduced here to increase the loading amount of g-C₃N₄. Compared to a g-C₃N₄ modified GCE, the g-C₃N₄/SiO₂ modified GCE displays a much higher ECL intensity. A high enhancement effect on the ECL intensity of g-C₃N₄/SiO₂ modified GCE is obtained in the presence of GSH in the electrolyte. Moreover, the enhanced ECL intensity shows a good linear relationship to the GSH concentration in the range from 1.0 × 10⁻⁷ to 5.0 × 10⁻⁴ M, with a detection limit of 2.0 × 10⁻⁸ M (6.1 ng/mL). Besides, the ECL sensor exhibits a good anti-interference ability and has been successfully applied in the detection of GSH in commercial samples. The proposed sensor provides a promising platform for life science.