Development of Tube Radial Distribution Chromatography Based on Phase-Separation Multiphase Flow Created via Pressure Loss

Abstract

A tube radial distribution chromatography (TRDC) method based on phase-separated multiphase flow created through phase transformation via temperature change has been developed. These systems typically required a temperature-controlling device containing a water bath and a stirrer. Herein, we proposed a novel TRDC system without a cooling device, where the phase transformation was achieved via pressure loss in a capillary tube of 50 μm inner diameter and 550 cm length. Model analytes were successfully separated with the developed TRDC system, which provided a simplified platform and helped to clarify the operating principle of TRDC based on phase transformation in a capillary tube.