Application of Molecularly Imprinted Polymer-modified Potentiometric Sensor for Quantitative Determination of Histamine in Serum

Abstract

A molecularly imprinted polymer-modified potentiometric histamine (HIS) sensor was prepared and used for quantitative determination of HIS in bovine serum. The calibration curve using the potential responses measured in 1 × 10⁻³ mol L⁻¹ phosphate buffer (pH 7.4) showed good linearity in the HIS concentration range of 3 × 10⁻⁴ to 1 × 10⁻² mol L⁻¹ (r = 0.92), with a detection limit of 1.6 × 10⁻⁴ mol L⁻¹. In bovine serum samples, the HIS sensor showed good recovery values of 91 – 104%. Therefore, this HIS sensor successfully determined the HIS concentration in bovine serum samples.