Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Rapid Communications
Fluorescence Enhancement Effect by Metal Nanoparticles-immobilized Microplate
Shuyi SUNKyohei MATSUISo TANABEDung NGUYENTakamasa KINOSHITAYojiro YAMAMOTOHiroshi SHIIGI
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Supplementary material

2020 Volume 36 Issue 7 Pages 787-789

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Abstract

In this reported work, we achieved high-throughput, highly sensitive fluorescent analysis using an enzyme-linked immunosorbent assay (ELISA) that employed a metallic nanoparticle (NP)-immobilized 96-well plate. The immobilization of metallic NPs on a 96-well plate effectively amplified fluorescent signals of the assay. The silver (Ag) NP-immobilized plate showed the best fluorescent enhancement effect of all plates immobilized by metal NPs. Our results demonstrate the potential of applying Ag NPs to enhance the efficiency of direct and indirect ELISA by the labeling of targets.

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© 2020 by The Japan Society for Analytical Chemistry
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