Abstract
A procedure is presented for determining isoprenaline (10-7-10-4M) in a flow injection system using chemiluminescence detection resulting from the reaction of isoprenaline with 10, 10′-dimethyl-9, 9′-biacridinium dinitrate (lucigenin) in aqueous solution of potassium hydroxide as a carrier stream. The logarithmic calibration curve was linear over the range from 1×10-7M to 1×10-4M. The effects of some analytical conditions on the chemiluminescence yield were investigated. The RSD at 1×10-4M isoprenaline is 0.9% (10 replicates). The method is directly applicable to aqueous solutions of isoprenaline injection (1mg/5ml).
