Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Rapid and Selective Sample Preparation for the Chromatographic Determination of Brassinosteroids from Plant Material Using Solid-Phase Extraction Method
Keiji GAMOHIkuyo YAMAGUCHISuguru TAKATSUTO
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1994 Volume 10 Issue 6 Pages 913-917

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Abstract

This report describes a rapid and selective method of sample preparation for the determination of brassinosteroids in samples of biological origin, based on solid-phase extraction. The steroids, brassinolide and castasterone, which possess a C-22, 23-diol group, were retained as their cyclic boronates formed on immobilized phenylboronic acid gel in a mixed solvent of pyridine and acetonitrile. After removal of impurities in the sample, parent brassinosteroids could easily be released from their boronates by a mixed solution of hydrogen peroxide and acetonitrile. The phenylboronic acid phase was shown to have a high affinity for brassinosteroids containing a C-22, 23-diol. The present method is a first example for the selective extraction of brassinosteroids in a biological sample using immobilized phenylboronic acid gel.

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