Abstract
p-Acetamidophenol analogs were proved to produce highly fluorescent compounds upon oxidation with peroxidase and hydrogen peroxide. The chemical structure of the major fluorescent product derived fromp-acetamidophenol was determined to be 5, 5′-diacetamido-2, 2′-bisphenol. A survey of 25 acetamidophenol analogs showed thatp- alkylamidophenols were suitable substrates for determining the peroxidase activity. In a region of low peroxidase activity in particular, the fluorescent intensity from p-acetamidophenol is more than twenty-times higher than that of 3- (4-hydroxyphenyl)propionic acid (HPPA), which is widely used as a fluorogenic reagent for determining peroxidase activity. The detection limit of peroxidase activity was 0.5μU/ml at a signal-to-noise (S/N) ratio of 3.
