-
Henry FREISER
1995 Volume 11 Issue 2 Pages
191-194
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
-
Masanobu SHIGA, Kenshi YAKATA, Masaaki AOYAMA, Kazumi SASAMOTO, Makoto ...
1995 Volume 11 Issue 2 Pages
195-201
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
p-Acetamidophenol analogs were proved to produce highly fluorescent compounds upon oxidation with peroxidase and hydrogen peroxide. The chemical structure of the major fluorescent product derived fromp-acetamidophenol was determined to be 5, 5′-diacetamido-2, 2′-bisphenol. A survey of 25 acetamidophenol analogs showed thatp- alkylamidophenols were suitable substrates for determining the peroxidase activity. In a region of low peroxidase activity in particular, the fluorescent intensity from p-acetamidophenol is more than twenty-times higher than that of 3- (4-hydroxyphenyl)propionic acid (HPPA), which is widely used as a fluorogenic reagent for determining peroxidase activity. The detection limit of peroxidase activity was 0.5μU/ml at a signal-to-noise (
S/N) ratio of 3.
View full abstract
-
Mari SHIMOKAWA, Riko YAMAZAKI, Toshio KINOSHITA
1995 Volume 11 Issue 2 Pages
203-208
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
The inhibitory effect of
α2-plasmin inhibitor (
α2PI) on plasmin (PLN) was found to be cancelled by a detergent having a long normal alkyl chain. Since the detergent did not affect the PLN activity, it should be useful for the determination of plasminogen (PLG) and PLN in plasma that contains a considerable amount of
α2PI. PLN generation from PLG by urokinase (UK) was not inhibited by plasma
α2PI when cetyltrimethylammonium bromide (C
16Me
3Br) was added into the reaction media.
16Me
3Br (0.015%) cancelled the effect of at least 5μg/ml of
α2PI in the sample solution. This concentration corresponds to 200μg/ml in plasma before dilution and it is about three times the
α2PI level in normal plasma. The standard curve of the PLG-UK system gave the linearity from 0 to at least 400μg/ml of PLG in plasma before dilution. This concentration is as twice the level in normal plasma. Accordingly, plasma PLG can be correctly determined by using this detergent for the reagent.
View full abstract
-
Tadashi SEGAWA, Takahiro OOIZUMI, Taichi MATSUBARA, Tamio KAMIDATE, Hi ...
1995 Volume 11 Issue 2 Pages
209-212
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
Purpurogallin carboxylic acid (PurA) was proposed as a highly sensitive chemiluminescent (CL) reagent for the detection of peroxidase. The blank signal of PurA was lower than that of its mother moiety, purpurogallin, thus resulting in a decrease in the detection limit of peroxidase. The calibration curves of horseradish peroxidase and
Arthromyces ramosus peroxidase with PurA were linear over the ranges from a detection limit of 20fmol to 5.0pmol and 10fmol to 5.0pmol, respectively. PurA CL was less subject to interference from diverse albumin than was luminol CL.
View full abstract
-
Yoshihiro NISHIDA, Eigo ITOH, Masayuki ABE, Hiroshi OHRUI, Hiroshi MEG ...
1995 Volume 11 Issue 2 Pages
213-220
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
A series of fluorescent carboxylic acids with a common skeleton of 1, 3-benzodioxole-4- or 5-carboxylic acid were prepared as racemates or in an optically active form. Their potential as chiral derivatizing agents was evaluated in terms of the HPLC and
1H-NMR analyses of D, L-amino acids. Every acid with a carboxyl group at C-4 showed high separation ability of original enantiomers in both the HPLC and the NMR analyses, while the C-5 isomer showed little separation. As the difference in the bulkiness between the two substituents at C-2 became wider, the separation by HPLC increased. These studies allowed us to develop new chiral reagents useful both for HPLC and
1H-NMR analyses, along with our previous reagent, (S)-TBMB carboxylic acid.
View full abstract
-
Akio YUCHI, Hitotoshi MURASE, Hiroko WADA
1995 Volume 11 Issue 2 Pages
221-226
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
Complexes of tetravalent metal ions with chromogenic chelating reagents having only one methyliminodiacetate group ortho to OH group were examined for spectrophotometric or fluorometric determination of fluoride. Zirconium(IV) and hafnium(IV) were superior to thorium(IV) as a central metal ion. Three triphenylmethane dyes showed comparable sensitivities. Although two azo dyes had lower blank values and higher sensitivities than triphenylmethane dyes, further modification involving enhancement of solubilities is required for practical use. Zirconium(IV) complex of a new fluorescent reagent derived from sulfonefluorescein showed an increase in fluorescence intensity on the reaction with fluoride. The mechanism of this change in spectroscopic properties is discussed.
View full abstract
-
Minoru TANAKA, Masanobu YOSHINAGA, Masahiro ITO, Hiroaki UEDA
1995 Volume 11 Issue 2 Pages
227-231
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
Cyclodextrin-bonded stationary phases on a silica support without an unreacted spacer chain for liquid chromatography were prepared by the reaction of hydride-modified silica with monoallyloxyethylated β- and γ-cyclodextrins. Both cyclodextrins were coupled to 3-(2-tosyloxyethoxy)propyl silica to produce stationary phases with an unreacted spacer chain. The cyclodextrin units in these four stationary phases were connected to the silica surface with the same linkage. The enantiomeric separation ability of these cyclodextrin stationary phases was investigated using eleven dansylamino acids. Both β-cyclodextrin stationary phases exhibited a comparable enantioselectivity, except for dansyl- DL-aspartic and -glutamic acids. However, the β-cyclodextrin stationary phase without an unreacted spacer chain gave a higher column efficiency, compared with the β-cyclodextrin stationary phase with an unreacted spacer chain. On the other hand, the γ-cyclodextrin stationary phase without an unreacted spacer chain was more enantioselective than that with an unreacted spacer chain. The effect of silanization of unreacted silanol groups on the silica surface is discussed.
View full abstract
-
Susana de MARCOS, Javier GALBÁ, Juan R. CASTILLO
1995 Volume 11 Issue 2 Pages
233-238
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
An enzymatic fluorometric method for the determination of lactate based on its oxidation to pyruvate by NAD in the presence of lactate dehydrogenase (LDH) is proposed. The method relies on a decrease in the enzyme fluorescence during the reaction, which can be ascribed to the stability constant difference between the NAD-LDH and NADH-LDH complexes. A theoretical model was developed in order to predict the effect of the LDH and NAD concentrations on the analytical signal; also, the figures of merit of the proposed method were determined. The method allows the determination of lactate in synthetic blood serum with no interference, provided the sample is previously deproteinated with perchloric acid.
View full abstract
-
Ming-Yu DING, Hitoshi KOIZUMI, Yoshihito SUZUKI
1995 Volume 11 Issue 2 Pages
239-243
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
Three chromatographic systems: ion exchange chromatography, ion exclusion chromatography and reversed phase chromatography, have been used for the simultaneous determination of organic acids in wine. The common organic acids were separated using all three chromatographic systems. When an ion exchange column (TSK gel IC Anion-PW) was used, organic acids and inorganic anions (Cl
- and SO
42-) in wine were determined simultaneously without interference. The sharp peaks were obtained when an ion exclusion column (TSK gel OApak-A) was employed. A rapid separation of organic acids has been achieved, within about 7min, when a reversed phase column (Zorbax ODS) was used. In ion exclusion and reversed phase systems combined with UV detection, however, other organic compounds which have ultraviolet absorption at 210nm interfered with the determination of organic acids when practical wine samples were analyzed. IC is an obvious alternative for determination of organic acids in wine.
View full abstract
-
Chiyo MATSUBARA, Yuta YOKOI, Maiko TSUJI, Kiyoko TAKAMURA
1995 Volume 11 Issue 2 Pages
245-249
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
A rapid and sensitive flow injection analysis (FIA) with spectrophotometric detection by a Ti(IV)-porphyrin reagent is described for the determination of oxalate in foods using an immobilized oxalate oxidase reactor to yield hydrogen peroxide. The reagent is an aqueous solution of oxo[5, 10, 15, 20-tetra(4-pyridyl)porphyrinato]titanium(IV), TiO(tpypH
4)
4+, which reacts with hydrogen peroxide to form a monoperoxo complex, TiO
2(tpypH
4)
4+, having an absorption peak at 450nm. The absorbance was proportional to the concentration of hydrogen peroxide. The flow-injection manifold comprised a two-channel system with an immobilized oxalate oxidase column. A linear relation was observed between the absorbance and the oxalate concentration ranging from 5×10
-7to 2.5×10
-4M (
r=0.999), and 25 samples could be analyzed in one hour. The relative standard deviation for ten replicate measurements was 0.48% at 2.5×10
-5M (500pmol/injection). The application of this system for the determination of oxalate in foods gave satisfactory results. A good correlation (
r=0.996) was seen between the results obtained by the proposed method and by the conventional method using a commercial F-kit.
View full abstract
-
Shoichiro YOSHIDA, Hiroshi KANNO, Tadashi WATANABE
1995 Volume 11 Issue 2 Pages
251-256
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
Amperometric
L-glutamic acid sensors were fabricated by depositing
L-glutamate oxidase (GLOD) and horseradish peroxidase (HRP) on the tin oxide surface by means of either encapsulation into electropolymerized pyrrole or sequential chemical modification via surface hydroxyl groups. After optimization for the mixing ratio of the two enzymes during polypyrrole (PPy) formation and for the thickness of the PPy film, the GLOD/HRP/PPy electrode gave a nearly linear response to
L-glutamic acid in the concentration range of 10
-7 to 10
-4M, with a response time of about 1min. The GLOD/HRP bilayer-modified electrode showed a sensitivity comparable to that of the PPy-encapsulated bienzyme electrode. Both type of electrodes exhibited excellent substrate specificity. The pH dependence and lifetime of the sensor response are also given.
View full abstract
-
Kunihiko YOKOI, Masayo MIZUMACHI, Tsutomu KOIDE
1995 Volume 11 Issue 2 Pages
257-260
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
A sensitive stripping voltammetry for the determination of cadmium, with an adsorptive accumulation of complexes on a hanging mercury drop electrode with Calcein Blue, 8-[N, N bis(carboxymethyl)aminomethyl]-4-methylumbelliferone, has been developed. After accumulation of the complex, the electrode potential is scanned with differential pulse modulation and the reduction current for cadmium is observed at -0.8V. Under optimum conditions (1μM Calcein Blue, pH 7.2, adsorption at -0.2V for 3min), the detection limit was 0.015nM. Interference from iron can be reduced by the addition of citrate ion. The method was applied to the determination of cadmium in fresh water samples.
View full abstract
-
P. SHARMA
1995 Volume 11 Issue 2 Pages
261-262
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
In view of the difference in the toxicity of As(III) and As(V), arsenic has been determined in terms of its oxidation state at trace levels in aqueous matrices by differential pulse polarography. A determination limit of 10μg/l was observed.
View full abstract
-
Hideyuki ITABASHI, Hiroshi KAWAMOTO, Norihiro NIIBE, Kin-ichi TSUNODA, ...
1995 Volume 11 Issue 2 Pages
263-265
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
Based on a kinetic aspect of the extraction of copper(II) with dithizone, a method for the simultaneous determination of copper(II) Complexing capacity of natural water and lability of soluble copper(II) complex was developed. The feasibility of the proposed method was confirmed using some chelating agents such as nitrilotriacetic acid, ethylenediaminetetraacetic acid and citrate instead of naturally occurring ligands. The proposed method was applied to river water samples. The copper(II) complexing capacity of the water sample from urban area was larger than that from up-stream, due to a ligand which originated from human activity.
View full abstract
-
Ryusuke KOBAYASHI, Keishichiro IMAIZUMI, Mitsuhiro KUDO
1995 Volume 11 Issue 2 Pages
267-269
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
A method has been developed for the determination of silicon in urine by graphite-furnace atomic absorption spectrometry. Nickel chloride was employed for the matrix modifier. The tolerable ashing temperature was raised to 2200°C in the presence of a matrix modifier. The urine was diluted 1+349 with distilled water, and introduced into a pyrocoated graphite tube together with a nickel solution. Recoveries of the silicon added to the urine sample ranged from 85.5 to 89.3%; the relative standard deviation was within 10% at the 10mg/l level. The silicon content, determined by the proposed method, ranged from 2.6-23.7mg/l.
View full abstract
-
Concepcion S. MENDOZA, Didarul A. CHOWDHURY, Satsuo KAMATA
1995 Volume 11 Issue 2 Pages
271-275
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
New xanthic acid derivatives containing the bisbutyl polymethylene group were used for silver extraction in 0.1M NaClO
4. Solvent extraction results showed that silver extraction was established through 1:2 (silver:ligand) complexes. The stabilities of the extractable complexes were determined based the length of the polymethylene chain between the donor atoms and the presence of a neighboring functional group within the structure of the ligands, as revealed by the
Kex values. The probable extraction mechanism (extraction and back-extraction) and interferences are also discussed.
View full abstract
-
Elwira LACHOWICZ
1995 Volume 11 Issue 2 Pages
277-280
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
The transport of gold ions (5×10
-5M) from a diluted hydrochloric acid solution to a stripping solution across a supported liquid membrane (SLM) containing 0.1M solution of polydentate neutral sulfide podand 1, 12-di-2-thienyl-2, 5, 8, 11- tetrathiadodecane (TTD) in m-chlorotoluene was studied. The rate of transport of gold ions increased in the sequence of the stripping solutions, NaBr_??_NH
3, thiourea<NaSCN<NaI<thiosulfate. Among three microporous polymers used, the rate with Celgard 2500 membrane is ca. 23% higher than for Celgard 4510 or 2502. The present result shows that the selectivity for gold ion is good against copper, zinc and iron ions present in 200:1 excess. The gold flux (J) increases almost linearly with an increase in the initial gold concentration over the range 10
-5-10
-4M; for 5×10
-5M Au it is equal to 10.3×10
-8mol cm
-2 h
-1.
View full abstract
-
Ashok KUMAR
1995 Volume 11 Issue 2 Pages
281-284
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
A spectrophotometric method for the determination of palladium, tellurium and iridium from nitric acid media after extraction of their 2-mercapto-4-methyl-5-phenylazopyrimidine complexes into molten naphthalene has been develop and possible synergistic effects have been investigated. Dioxane, methanol, ethanol, 1-propanol, acetone and acetonitrile are used as the organic component of the mixed (polar) phase; the maximum enhancement is obtained with acetonitrile. Solid naphthalene containing the metal complex is separated by filtration and dissolved in chloroform; trace amounts of these metals are determined spectrophotometricaly. Beer′s law is obeyed in the following concentration ranges: Pd, 7.0-84.0μg; Te, 10.0-125.0μg and Ir, 6.0-90.0μg/10ml of the final solution. The molar absorptivities (dm
3 mol
-1 cm
-1) and Sandell′s sensitivities (μg cm
-2) were calculated: Pd(II)=2.05×10
4 and 0.0052, Te(IV)=1.84×10
4 and 0.0069 and Ir(III)=0.95×10
4 and 0.020, respectively. Ten replicate analyses containing 12.5μg of Pd(II), 21.0μg of Te(IV) and 85.0μg of Ir(III) gave mean absorbances of 0.241, 0.302 and 0.420 with relative standard deviations of 0.53, 0.36 and 0.85%, respectively. The interference of various ions was studied, and conditions were developed for the determination of these metals in certain alloys and synthetic mixtures.
View full abstract
-
Toshiaki HATTORI, Masanao KATO
1995 Volume 11 Issue 2 Pages
285-287
Published: 1995
Released on J-STAGE: July 27, 2007
JOURNAL
FREE ACCESS
-
Hiroshi KAWAMOTO, Hideyuki ITABASHI, Mamoru NEMOTO
1995 Volume 11 Issue 2 Pages
289-290
Published: 1995
Released on J-STAGE: July 27, 2007
JOURNAL
FREE ACCESS
-
Hiroshi KAWAMOTO, Hideyuki ITABASHI, Hideo AKAIWA
1995 Volume 11 Issue 2 Pages
291-293
Published: 1995
Released on J-STAGE: July 27, 2007
JOURNAL
FREE ACCESS
-
Yuta YASAKA, Takashi MATSUMOTO, Minoru TANAKA
1995 Volume 11 Issue 2 Pages
295-297
Published: 1995
Released on J-STAGE: July 27, 2007
JOURNAL
FREE ACCESS
-
Akira SANO, Shoji TAKITANI, Hiroshi NAKAMURA
1995 Volume 11 Issue 2 Pages
299-301
Published: 1995
Released on J-STAGE: July 27, 2007
JOURNAL
FREE ACCESS
-
Kunishige NAITO, Yasukiyo EJIRI, Sadaaki MORIGUCHI, Shinsuke TAKEI
1995 Volume 11 Issue 2 Pages
303-305
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
-
Koichi YAMAMOTO, Takahiro KUMAMARU
1995 Volume 11 Issue 2 Pages
307-310
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
-
Tomonori HOSHI, Hiroki TAKESHITA, Jun-ichi ANZAI, Tetsuo OSA
1995 Volume 11 Issue 2 Pages
311-312
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
-
Xian Ren CHEN, Yuqi FENG, Hisanori IMURA, Kazuhisa HIRATANI, Kousaburo ...
1995 Volume 11 Issue 2 Pages
313-315
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
-
Kei TODA, Tetsuo SHIMIZU, Isao SANEMASA
1995 Volume 11 Issue 2 Pages
317-318
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
-
Shiquan TAO, Yasuaki OKAMOTO, Takahiro KUMAMARU
1995 Volume 11 Issue 2 Pages
319-322
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
-
M. S. El-SHAHAWI, M. M. KAMAL
1995 Volume 11 Issue 2 Pages
323-326
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
-
Sergey B. SAVVIN, Alla V. MIKHAILOVA, Vladimir V. KUZNETSOV, Veronica ...
1995 Volume 11 Issue 2 Pages
327-328
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
-
Sohair A. El-REEFY, Mohamed R. El-SOUROUGY, Eman A. El-SHERIF, Hisham ...
1995 Volume 11 Issue 2 Pages
329-331
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS
-
Masataka HIRAIDE, Zuo-Sheng CHEN, Hiroshi KAWAGUCHI
1995 Volume 11 Issue 2 Pages
333-335
Published: April 10, 1995
Released on J-STAGE: July 04, 2006
JOURNAL
FREE ACCESS