Determination of Peroxidase with a Chemiluminescence Reaction of Homogentisic Acid γ-Lactone in Non-Aqueous Media

Tadashi SEGAWA; Takahiro OOIZUMI; Teruki YOSHIMURA; Masahiko TOHMA; Tamio KAMIDATE; Hiroto WATANABE

doi:10.2116/analsci.11.581

Tadashi SEGAWA, Takahiro OOIZUMI, Teruki YOSHIMURA, Masahiko TOHMA, Tamio KAMIDATE, Hiroto WATANABE

著者情報

キーワード: Chemiluminescence, peroxidase, taurocholic acid, homogentisic acid γ-lactone, enzyme immunoassay

ジャーナルフリー

1995 年 11 巻 4 号 p. 581-586

DOI https://doi.org/10.2116/analsci.11.581

詳細

抄録

Chemiluminescence (CL) was observed during the peroxidation of homogentisic acid γ-lactone catalyzed by horseradish peroxidase (HRP) in an aqueous dimethyl sulfoxide (20%v/v) solution. The intensity of light emission was linearly correlated with the HRP concentration over the range from the detection limit of 1fmol to 30pmol. The CL reaction was applied to the detection of HRP-labeled antigen in an enzyme immunoassay of taurocholic acid. Taurocholic acid was determined in the range from 10pg to 100ng. The detection limit with the present method was 25-times lower than that with a colorimetric method.

責任著者(Corresponding author)

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