Determination of the Free Fatty Acid Content in Fats and Oils by Flow Injection Analysis with Electrochemical Detection

抄録

A flow injection analytical method using a walljet-type electrochemical detector was developed for determining the content of free fatty acids. A simple flow injection system was constructed. An ethanol solution containing 3mM 2-methyl-1, 4-naphthoquinone and 38mM LiClO₄ served as the carrier solution. The flow-signal response for higher fatty acids was linear between 25 and 1500pmol test^-1. The free fatty acid content in various fat and oil samples was determined by both the present and conventional titration methods. The present method was found to be not only highly sensitive with high reproducibility, but also quite simple and rapid.