抄録
Characteristics of a column with continuously varying properties were investigated. The velocity change was induced by column′s geometry, with a continuously varying diameter inside the column. This column was compared to a standard analytical column and to a narrower classical column in view of separation of acyclovir from the human plasma samples. Typical performance of all columns operating under the same conditions was examined. The proposed column configuration reduces retention times compared to widely used analytical columns. Compared to the standard column, a higher linear velocity at the same flow-rate was achieved. Therefore, the retention times are shorter andthe consumption of the mobile phase was smaller.
