Abstract
A high performance liquid chromatographic determination of tranexamic acid and two vitamins, i.e., vitamin U chloride and anthranilic acid, with chemiluminescence detection was investigated. o-Phthalaldehyde(OPA) solution which contained 2-mercaptoethanol was used as a fluorescent labeling reagent. OPA solution was prepared by mixing OPA and 2-mercaptoethanol in ethanol, and then diluting with borate buffer solution. Bis(2, 4, 6-trichlorophenyl)oxalate and hydrogen peroxide were used a postcolumn chemiluminescent reagent. The intensity of the chemiluminescence was in the order of tranexamic acid> vitamin U chloride>anthranilic acid. The calibration curves for the compounds were linear at least in the range from 1x10-7M to 1x10-4M. The detection limits for tranexamic acid, vitamin U chloride and anthranilic acid were 5x10-8M, 4x10-7M and 7x10-7M, respectively. The relative standard deviation was less than 9%(n=5). The proposed method was applied to the determination of tranexamic acid and vitamin U chloride in commercially available medicine.
