Chemiluminogenic reaction of Arg-containing peptides with benzoin or its analogues

抄録

Novel Chemiluminogenic reaction is described for the sensitive determination of Arg-containing peptides. The reaction is based on fluorescence derivatization of the peptides with benzoin in a potassium hydroxide solution at 100°C for 2min, followed by chemiluminescence reaction with an aprotic polar solvent (N, N-dimethylformamide, dimethyl sulfoxide or acetonitrile) in the presence of hydrogen peroxide. The reaction conditions were established by employing Arg. Furthermore, fifteen kinds of benzoin analogues were examined under similar reaction conditions. Among them, benzoin, desyl bromide and 2′-chloro-3, 4-dimethoxybenzoin gave same levels of chemiluminescence intensities for Arg. These reagents are useful as chemilum- inogenic derivatization reagents for Arg-containing peptides as well as guanidino compounds; the detection limits are 5-20pmol/ml in the reaction mixture for the peptides under the established reaction conditions.