1986 Volume 2 Issue 1 Pages 81-85
A sensitive and specific high performance liquid chromatographic method for the simultaneous determination of cyanate and Carbamyl phosphate was established. The chromatographic conditions were as follows: column, TSK gel SAX (4mm I.D.×50mm); eluent, 0.075M NaCl; column temperature, 20°C. The detection was based on the indophenol reaction of ammonia which was released by the hydrolysis of cyanate or carbamyl phosphate. To suppress their hydrolysis during the sample preparation, the deproteinization of biological samples with trichloroacetic acid was carried out at 0°C, and the interval between addition of trichloroacetic acid and neutralization was restricted to 20s. Taking advantages of the sensitivity and precision of the present method, we developed a procedure that permits measurement of concentrations of cyanate as low as 0.8μM and those of carbamyl phosphate as low as 0.5μM in samples of whole blood and liver extract.
