抄録
A simple and sensitive fluorometric method for determination of sulfide ion was developed. Sulfide ion gave a blue fluorescence (λex 338nm and λem 450nm) after alkylation reaction with methyl p-toluenesulfonate in dilute alkaline medium and the successive condensation reaction with taurine and o-phthalaldehyde at pH 8.0. Sulfide ion can be determined in the range 0.03-5nmol/100μl with relative standard deviations (n=10) of 1.6% at 0.2nmol and 2.7% at 3nmol. A 100-fold molar amount of each of common anions did not interfere the analysis, except cyanide, thiosulfate, sulfite and carbonate, the tolerance ratios of which were 5, 10, 30 and 50, respectively. Interferences caused by metal ions such as calcium(II ), magnesium(II) and zinc(II) can be removed by addition of EDTA, but copper(II) and manganese(II) must be absent.
