Electrogenerated Chemiluminescence Detector for Flow Injection Analysis

抄録

A new kind of detector system based on electrogenerated chemiluminescence (ECL) was devised for flow injection analysis. The flow through type of electrolytic cell was modified to observe the ECL. The quartz optical window was set in front of the working electrode (Pt plate). The observed luminescence intensity was amplified by using a lock-in amplification technique. The preliminary examination of the whole system was performed using luminol with a strong ECL. The injection of a sample solution in a carrier solution of sodium carbonate caused a peak-shaped luminescence at the electrode. Next the determination was performed for non-luminescent compounds after the pre-labeling with luminol. It was found that oligo peptides or bovine serum albumin can be determined by ECL method. Though, the sensitivity and the reproducibility of ECL method are almost comparable to existing methods, it has the advantage that two kinds of quantities, the luminescence intensity and the electrolytic current, can be obtained at the same time.