Determination of Iodide and Sulfide as p-Nitrobenzyl Derivatives by High Performance Liquid Chromatography

抄録

A new technique is presented for the determination of iodide and sulfide concentrations in aqueous solutions. The method is based on the ease with which the inorganic anions can be converted into their p-nitrobenzyl derivatives with p-nitrobenzyl bromide. The resulting derivatives are subsequently determined by high performance liquid chromatography with a 5-μm ODS separation column and ultraviolet (254nm) absorption detection. By this method, iodide and sulfide could be determined in the concentration ranges of 0.2-2.0mM and 60-600μM, respectively. Also discussed are the effects of added acid or base, reaction temperature, concentration of p-nitro-benzyl bromide used as the derivatizing reagent and reaction time.