Determination of S-Carbamyl Group in Biological Materials
1987 年 3 巻 1 号 p. 59-62
詳細
1987 年 3 巻 1 号 p. 59-62
A sensitive and simple method for the determination of acid soluble and acid insoluble S-carbamyl groups in biological materials is presented. This method is based on the chemical properties of the S-carbamyl group, which is stable in acidic solution and liberates cyanate in alkaline solution, and those of the cyanate analyzer reported previously. Each sample solution was mixed with 20%(w/v) trichloroacetic acid (TCA) solution; the mixture was incubated for 1h at 37°C, followed by centrifugation. For the determination of acid soluble S-carbamyl group, the supernatant was added to an equal volume of 1.0M NaOH. Liberated cyanate was determined by cyanate analyzer. For the determination of acid insoluble S-carbamyl group, the precipitate was washed twice with 7%(w/v) TCA solution and then dissolved in 1.0M NaOH. After ultrafiltration, cyanate in filtrate was determined. The usefulness and practicality of the present method were verified by applications to the determination of S-carbamyl group in liver, plasma and erythrocytes of mouse.
