Fluorometric Determination of Heparin with Dansylprotamine

Noriko NAKAMURA; Fumio IINUMA; Toshio KINOSHITA

doi:10.2116/analsci.3.175

Noriko NAKAMURA, Fumio IINUMA, Toshio KINOSHITA

著者情報

キーワード: Heparin determination, dansylprotamine, cellulose sulfate determination, dextran sulfate determination

ジャーナルフリー

1987 年 3 巻 2 号 p. 175-177

DOI https://doi.org/10.2116/analsci.3.175

詳細

抄録

Heparin was found to enhance the fluorescence of dansylprotamine. This finding was applied to microdeter- mination of the sulfated polysaccharides. The standard curves for heparin, cellulose sulfate and dextran sulfate were linear in the range of final concentration of 0.5 to 10.0μg/ml. Sulfated polysaccharides and dansylprotamine were found to bind each other through both electrostatic and hydrophobic interactions.

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