Simultaneous Determination of Triglycerides and β-D-Glucose by Flow Injection Analysis Using Enzyme Immobilized Open Tubular Reactors

抄録

Triglycerides of clinical importance were determined by a flow injection analytical (FIA) method using whisker- walled open tubular reactors, on the inner wall of which lipoprotein lipase and glycerol dehydrogenase were immobilized. Triglycerides were measured by monitoring the resulting NADH fluorimetrically. Since all the reactions were carried out in a flowing stream, pretreatments of samples prior to analysis could be reduced to a minimum. Completion of hydrolysis of triglycerides required more than 10min, so the stopped-flow procedure was adopted at this stage. Before the peak corresponding to triglycerides appears, a glucose dehydrogenase immobilized reactor was placed as the 2nd channel in a splitting-confluence FIA system to determine another species of clinical importance, β-D-glucose; triglycerides and β-D-glucose in the concentration ranges of clinical significance could be determined simultaneously with satisfactory reproducibility.