Sensitive Determination of Nitroprusside in Blood by High Performance Liquid Chromatography

Toshinori WATANABE; Yuriko NAGAMINE; Toshihiko TOIDA; Ichiro KOSHIISHI; Toshio IMANARI

doi:10.2116/analsci.4.203

Toshinori WATANABE, Yuriko NAGAMINE, Toshihiko TOIDA, Ichiro KOSHIISHI, Toshio IMANARI

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Keywords: High performance liquid chromatography, nitroprusside, cyanide, thiocyanate, biological materials

JOURNAL FREE ACCESS

1988 Volume 4 Issue 2 Pages 203-206

DOI https://doi.org/10.2116/analsci.4.203

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Abstract

A highly sensitive method for the determination of nitroprusside was developed by high performance liquid chromatography using an anion exchange column. In this method, nitroprusside was degraded to produce cyanide by heating in the presence of dithiothreitol, and the released cyanide was detected fluorometrically. A calibration curve for nitroprusside was linear in the range of 0.4 to 400pmol. The usefulness and practicability of the present method were verified by application to the determination of nitroprusside in rat blood, which was infused to control the blood pressure.

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