Isotachophoretic Examination of Interaction of Intercalators with Ribodinucleoside Monophosphates

Abstract

The isotachophoretic behavior of ribodinucleoside monophosphates (XpY) was studied in aqueous solution in the presence of intercalators under neutral pH conditions. The interaction of intercalators with the ribodinucleoside monophosphates was studied by the electrophoretic retardation of the nucleotides. Among the acridine-type intercalators, proflavine and quinacrine interacted favorably with ribodinucleoside monophosphates following the order CpG>GpC>UpA, while 9-aminoacridine interacted in the order UpA>GpC_??_CpG. Anthraquinone-type intercalators behaved similarly, among these, 1, 5-bis[(diethylaminoethyl)amino]anthraquinone interacted with the nucleotides more strongly than did 1, 8-bis[(diethylaminoethyl)amino]anthraquinone. Use of isotachophoresis is discussed as a rapid and convenient method for the characterization of DNA intercalators.