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Volume 4 (1988)
- Issue 6 Pages 541-
- Issue 5 Pages 439-
- Issue 4 Pages 337-
- Issue 3 Pages 233-
- Issue 2 Pages 121-
- Issue 1 Pages 3-
Volume 4, Issue 3
Displaying 1-21 of 21 articles from this issue
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Osamu YAMAMOTO, Kazuo SOMENO, Nobuhide WASADA, Jiro HIRAISHI, Kikuko H ...1988 Volume 4 Issue 3 Pages 233-239
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSAn integrated spectral data base system named SDBS was constructed, in which six kinds of spectral data, i.e., data of IR, MS, 1H-NMR, 13C-NMR, ESR and Raman spectra were compiled in an integrated way under the control of a directory data base. The spectra data are full pattern data and/or numerical data depending on the features of the spectra, most of which were obtained in our laboratory. Three options are provided as mode of access. In LOOK option the spectra for a given compound are displayed; in SEARCH option the identification of unknown compounds as well as the estimation of NMR chemical shifts and/or of the sub-structure of a molecule are possible; and in SIMULATE option, 1H-NMR and ESR spectra are generated from the NMR and ESR parameters, respectively. At present, approximately 17000 compounds are registered in the directory data base.View full abstractDownload PDF (448K) -
Tokuo MIZUNO, Koushi AZUMA, Kiichiro OTSUKA1988 Volume 4 Issue 3 Pages 241-246
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSA new moving belt type LC/MS interface (ITF) combining with fast atom bombardment (FAB) ionization has been developed. Unlike the conventional endless belt type ITF, this ITF employs a disposable belt. Furthermore, this incorporates a double belt structure that covers the sample surface with another belt when passing through the differentially pumped region. The performance of LC/MS with this ITF was investigated. This ITF has completely eliminated the problems of memory effect so far encountered. By applying this ITF to the analysis of cyclodextrin mixture, chromatograms and mass spectra based on FAB ionization were obtained.View full abstractDownload PDF (353K) -
Jun-ichi ANZAI, Jun-ya HASHIMOTO, Tetsuo OSA, Tadayuki MATSUO1988 Volume 4 Issue 3 Pages 247-250
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSThe gate surface of an ion-sensitive field effect transistor (ISFET) was covered with a penicillinase-adsorbed Langmuir-Blodgett (LB) membrane in order to examine the potentiometric response to H+ ion and penicillin G. The LB membrane-modified ISFET exhibited pH response of -30--40mV/pH, whereas near-Nernstian response was obserbed for the ISFET without LB membrane. The ISFET penicillin sensor showed the potentiometric response to penicillin G at milimolar level in 5mM phosphate buffer. The penicillin sensor could be used to determine penicillin G for more than 12 days without any deterioration.View full abstractDownload PDF (216K) -
Kunihiko DOHTSU, Shigeori TAKENAKA, Naotoshi NAKASHIMA, Makoto TAKAGI1988 Volume 4 Issue 3 Pages 251-254
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSThe isotachophoretic behavior of ribodinucleoside monophosphates (XpY) was studied in aqueous solution in the presence of intercalators under neutral pH conditions. The interaction of intercalators with the ribodinucleoside monophosphates was studied by the electrophoretic retardation of the nucleotides. Among the acridine-type intercalators, proflavine and quinacrine interacted favorably with ribodinucleoside monophosphates following the order CpG>GpC>UpA, while 9-aminoacridine interacted in the order UpA>GpC_??_CpG. Anthraquinone-type intercalators behaved similarly, among these, 1, 5-bis[(diethylaminoethyl)amino]anthraquinone interacted with the nucleotides more strongly than did 1, 8-bis[(diethylaminoethyl)amino]anthraquinone. Use of isotachophoresis is discussed as a rapid and convenient method for the characterization of DNA intercalators.View full abstractDownload PDF (276K) -
Tatsumu MORIMOTO, Shigeyuki OCHIAI, Tatsuya SEKINE1988 Volume 4 Issue 3 Pages 255-259
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSSolvent extractions of copper(II) from aqueous thiocyanate solutions into carbon tetrachloride containing benzoyltrifluoroacetone and into 4-methyl-2-pentanone were studied. From the data of the former extraction system, the stability constants of metal thiocyanate complexes in the aqueous phase were determined. From the data of the latter system, the extraction constants for the CuSCN+ClO4-. Cu(SCN)2 and Na+Cu(SCN)3- species were obtained. The extraction curve of this metal ion as well as those of nickel(II), cobal (II), zinc(II), cadmium(II), mercury(II) and lead(II) in this liquid-liquid system were considered from the standpoint of the stability and extractability of the complexes.View full abstractDownload PDF (273K) -
Shizuo FUJIWARA, Nobutaka YOSHIKUNI, Nobuaki KONDO, Rokuro KURODA1988 Volume 4 Issue 3 Pages 261-265
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSNon-aqueous solvent extraction of acid in papers is described; the process employs a liquid ion-exchanger in chloroform. The amounts of the acid in the so-called acid paper are determined by the normal method of aqueous extraction and by the organic solvent extraction and the values are compared. The results of investigation confirm that the acid content of the acid paper must be determined by the non-aqueous solvent extraction. It is pointed out that the level of the acidification of the "acid paper" can not be presented in terms of the value of pH, as is usually done, but instead should be presented in terms of the amount of substance(mol) of acid per gram of paper. Because the analysis of the "acid paper" must be carried out on a nondestructive basis, the upside-down glass electrode pH meter is recommended as a conventional tool for the nondestructive determination of the acidity of papers of precious books. Examples of the latter case are shown.View full abstractDownload PDF (336K) -
Tomozo KOH, Yasuyuki MIURA, Mikio SUZUKI1988 Volume 4 Issue 3 Pages 267-271
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSA spectrophotometric method has been developed for the determination of tetrathionate, thiosulfate and sulfide in mixtures. The conditions for tetrathionate to be stoichiometrically converted to thiosulfate were established by varying reaction time, reaction temperature, pH and amount of sulfide. The present method consists of three procedures: I, II and III. The absorbance obtained by Procedure I corresponds to the sum of the thiosulfate and twice the tetrathionate in the mixture. The absorbance obtained by Procedure II, where tetrathionate was not sulfitolyzed and sulfide was removed, corresponds to the amount of thiosulfate only. The absorbance obtained by Procedure III corresponds to the sum of the amount of thiosulfate and twice that of sulfide. The method is applicable to the determination of tetrathionate, thiosulfate and sulfide in amounts more than 0.1μmol and gave a relativestandard deviation of 1.2% at 1μmol level of tetrathionate in the presence of 1μmol of sulfide and 2μmol of thiosulfate.View full abstractDownload PDF (347K) -
Norinobu YONEHARA, Shinichi AKAIKE, Hayao SAKAMOTO, Masaakira KAMADA1988 Volume 4 Issue 3 Pages 273-276
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSA flow injection analytical method is proposed for the spectrophotometric determination of trace amounts of bromide based on its catalytic effect on the oxidation of Pyrocatechol Violet by hydrogen peroxide in strongly acidic solution. Bromide in water samples can be determined at a sampling rate of 45h-1 with 500μl sample injections. The detection limit is 10μg l-1 and the calibration graph for bromide is linear over the range of 10-600μg l-1. The relative standard deviations are 2.5% for 20μg l-1 bromide and 1.4% for 105μg l-1 bromide. The procedure is applied successfully to the determination of bromide in natural waters.View full abstractDownload PDF (226K) -
Yuzo TAMARI, Akihiro TAKADA, Haruo TSUJI, Yuzuru KUSAKA1988 Volume 4 Issue 3 Pages 277-280
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSMorin (5, 7, 2′, 4′-tetrahydroxyflavonol) forms a stable chelate with thorium(IV). The chelate showed a constant fluorescence intensity (λex 415nm, λem 505nm) in acidic solutions, pH 2.2. The stoichiometric composition of the chelate was 1:1 (thorium:morin). In the presence of arsenic(V), the chelate dissociated, owing to the formation of a more stable complex of thorium arsenate. By using the experimental result that the fluorescence intensity of thorium-morin chelate decreased in proportion to the concentration of arsenic(V), 0.01-1ppm of arsenic(V) could be determined. In the reaction, arsenic(III) did not form a thorium arsenite complex. Accordingly, arsenic(V) in the mixture of arsenic(III) and arsenic(V) was accurately determined by the fluorescence quenching method.View full abstractDownload PDF (211K) -
Kunihiko AKATSUKA, Naoki NOBUYAMA, Ikuo ATSUYA1988 Volume 4 Issue 3 Pages 281-285
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSA highly sensitive method was developed for the determination of cadmium, lead and zinc in natural waters by atomic absorption spectrometry after coprecipitation with 8-quinolinol. Quantitative recoveries of the metals were possible over the pH range 8-10 when magnesium was present as a carrier ion. The precipitates thus formed were analyzed directly by an atomic absorption spectrometer equipped with a specially deviced graphite furnaceand a miniature cup. Reliability of this procedure was confirmed by the determination of the metals in lake and
river water samples. For the analysis of a 400-cm3 portion of water sample, detection limits(3σb) for Cd(II), Pb(II)
and Zn(II) are 0.6, 2.3 and 1.9ng dm-3, respectively. A corresponding precision of 3-5% was typical.View full abstractDownload PDF (305K) -
Kenji MOTOJIMA, Katsuyoshi TATENUMA, Kouichi UCHINO, Akira YONETANI1988 Volume 4 Issue 3 Pages 287-291
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSA new and simple method for the determination of ppt (ng/l) levels of cobalt in aqueous samples is described. The method involves the extraction of cobalt into m-xylene as its 2-nitroso-1-naphthol Chelate, followed by elimination of the other metals and the excess reagent extracted in the m-xylene by the successive washing with hydrochloric acid and sodium hydroxide solution. Finally the cobalt in the m-xylene is determined by graphite furnace atomic absorption spectrometry. Since the formation and extraction properties of cobalt 2-nitroso-naphthol chelate are specific and selective, the resulting m-xylene contains only the cobalt chelate. More than 2ng of cobalt in 1l of aqueous sample (2ppt) can be accurately determined without the effects of many other substances.View full abstractDownload PDF (289K) -
Muneyuki KOHRI, Osamu KUJIRAI, Kei YAMADA, Haruno OKOCHI1988 Volume 4 Issue 3 Pages 293-297
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSSingle-column anion exchange chromatography has been combined with inductively coupled plasma-atomicemission spectrometry (ICP-AES). A complex Ni-base or Co-base heat-resistant alloy was dissolved in a mixed acid containing hydrofluoric acid. A strongly basic anion exchange resin was used to separate the alloying elements. Hydrofluoric acid was removed by fuming or was masked after elution in order to use a glass sample introduction system for the ICP-AES. Thirteen alloying elements were determined in Ni-base and eleven in Co-base heat-resistant alloys. Good results were obtained by both the calibration curve method and the internal standard method. Synthetic standard solutions were used for the calibration. Magnesium was used as an internal standard element.View full abstractDownload PDF (333K) -
Hiroaki TAO, Akira MIYAZAKI, Kenji BANSHO1988 Volume 4 Issue 3 Pages 299-302
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSThe determination of Be in natural water by gas chromatography-helium microwave induced plasma emission spectrometry is described. Beryllium was concentrated about 230 times by the extraction with acetylacetone into chloroform and by further evaporation of the solvent. A batchwise ligand vapor method was used to prevent any beryllium acetylacetonate from remaining in the gas chromatographic column. The detection limit was 10pg in 30ml of water sample and the relative standard deviation was 4.1% at 10ng of Be. The method was applied to the determination of Be in lake water samples.View full abstractDownload PDF (265K) -
Masayuki KUNUGI, Mamoru MURATA1988 Volume 4 Issue 3 Pages 303-306
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSCarbon concentration in aerosol samples was determined by X-ray fluorescence spectrometry using a synthetic multilayers crystal (W/C, 2d=16.0nm) as an analyzer. X-Ray fluorescence is an excellent method because of its handiness and non-destructive nature as well as the simultaneous determination of a number of elements with low and high atomic numbers in a single sample. Carbon concentration in urban aerosols less than 10μm in diameter varies widely depending upon the sources and the seasons. This study reports that the carbon concentration in aerosol samples can be determined by X-ray spectrometer over a wide concentration range.View full abstractDownload PDF (209K) -
Takeo SATO, Toyoaki KATO1988 Volume 4 Issue 3 Pages 307-311
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSThe elemental abundances in the International Atomic Energy Agency standard reference material Horse Kidney (H-8) were determined in the framework of the research project on Intercomparison of Cadmium and Other Elements in IAEA Horse Kidney. Instrumental thermal-neutron and photon activation analyses were applied to the mufti-element determination in this standard material. This material was then tested for use as a comparative standard for the mufti-element analysis of related biological materials; human liver and kidney tissues. The National Bureau of Standards Orchard Leaves and Bovine Liver were also used as comparative standards. Merits and comments are given on the basis of their applicability and versatility as multi-element reference materials.View full abstractDownload PDF (363K) -
Tomonori HAYASHI, Shunji SAKAMOTO, Toru FUWA, Ikue WADA (nee MORITA), ...1988 Volume 4 Issue 3 Pages 313-316
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSA high performance liquid chromatographic method for the determination of human epidermal growth factors (hEGFs) in human urine is described. Using an anti-hEGF antibody column as a precolumn and an ODS column as an analytical column, each urine sample was directly injected onto the precolumn with aqueous mobile phase. With 0.2% trichloroacetic acid solution, the trapped hEGFs were transferred to the analytical column to allow their separation by reversed phase mode. The present method revealed that human urine contains hEGF[1-50], [1-51] and [1-52] in addition to hEGF[1-53]; the content determined by native fluorescence detection coincided with that by enzyme immunoassay. In the present method, the relative standard deviation (RSD) was less than 3% (n=6) for 25ng/ml of hEGF[1-53] and the detection limit was ca. 3ng for each hEGF, and the loading capacity of hEGF[1-53] on the anti-hEGF antibody precolumn was unchanged during analyses of 500 human urine samples.View full abstractDownload PDF (263K) -
Fumio IINUMA, Masayoshi TABARA, Makiko SUZUKI, Mitsuo WATANABE1988 Volume 4 Issue 3 Pages 317-320
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSThe fluorescence of xanthurenic acid was found to be greatly enhanced after heating in the methanol solution of 25%(v/v) diethylamine and 3mM calcium nitrate. This finding was successfully applied to the assay procedure of xanthurenic acid in urine. The fluorescence intensity of xanthurenic acid by the calcium nitrate-diethylamine method was measured at 485nm with excitation at 375nm. The calibration curve for xanthurenic acid showed a linear relation in the range of 50pmol/ml to 1.5nmol/ml. The relative standard deviation at 1.5nmol was 2.9% (n=6). In this procedure, recovery of xanthurenic acid in the five samples of urine was found to be 102.0±6.2% (n=5). Xanthurenic acid contents in urine of healthy adults which were collected for 24h were 12.4±2.1μmol/d (male, 39 years old, n=5) and 7.5±1.9μmol/d(female, 31 years old, n=5).View full abstractDownload PDF (194K) -
Kin-ichi TSUNODA, Akira NOMURA, Joseph YAMADA, Sueo NISHI1988 Volume 4 Issue 3 Pages 321-323
Published: 1988
Released on J-STAGE: July 27, 2007
JOURNAL FREE ACCESSDownload PDF (173K) -
Yoshito WAKUI, Hideyuki MATSUNAGA, Toshishige M. SUZUKI1988 Volume 4 Issue 3 Pages 325-327
Published: 1988
Released on J-STAGE: July 27, 2007
JOURNAL FREE ACCESSDownload PDF (145K) -
Tamio KAMIDATE, Atsushi KATAYAMA, Hiroto WATANABE1988 Volume 4 Issue 3 Pages 329-330
Published: 1988
Released on J-STAGE: July 27, 2007
JOURNAL FREE ACCESSDownload PDF (112K) -
Junichi TAKAHASHI, Reinosuke HARA1988 Volume 4 Issue 3 Pages 331-333
Published: June 10, 1988
Released on J-STAGE: June 27, 2006
JOURNAL FREE ACCESSDownload PDF (156K)
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