High Performance Liquid Chromatography of Amino Acids and Peptides on Strong Cation-Exchange Polymer Gel

Tadao KONISHI; Masafumi KAMADA; Hiroshi NAKAMURA

doi:10.2116/analsci.5.667

Tadao KONISHI, Masafumi KAMADA, Hiroshi NAKAMURA

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Keywords: High performance liquid chromatography, cation-exchange polymer gel, peptide, amino acid, hydrophobic interaction, fluorometric detection

JOURNAL FREE ACCESS

1989 Volume 5 Issue 6 Pages 667-670

DOI https://doi.org/10.2116/analsci.5.667

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Abstract

The separation of amino acids and peptides on a strong cation-exchange polymer column (Polyspher PE A) has been investigated. Retentions of hydrophobic amino acids and peptides were affected by hydrophobic interaction with the polymer matrix, which was useful for separation of peptides with close pl values. Simultaneous separation of acidic, neutral and basic peptides was successfully performed by linear gradient elution.

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