Determination of 7-Dehydrocholesterol in Human Skin Surface Lipids by High Performance Liquid Chromatography with Fluorescence Detection

Tetsuharu IWATA; Hiroshi HANAZONO; Masatoshi YAMAGUCHI; Masaru NAKAMURA; Yosuke OUHKURA

doi:10.2116/analsci.5.671

Tetsuharu IWATA, Hiroshi HANAZONO, Masatoshi YAMAGUCHI, Masaru NAKAMURA, Yosuke OUHKURA

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Keywords: 7-Dehydrocholesterol, high performance liquid chromatography, fluorescence detection, 3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl azide, human skin surface

JOURNAL FREE ACCESS

1989 Volume 5 Issue 6 Pages 671-673

DOI https://doi.org/10.2116/analsci.5.671

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Published: December 10, 1989 Received: July 10, 1989 Available on J-STAGE: June 30, 2006 Accepted: August 30, 1989 Advance online publication: - Revised: -
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Date of correction: June 30, 2006 Reason for correction: - Correction: ABSTRACT Details: Wrong : A simple, highly sensitive, high performance liquid chromatographic method with fluorescence detection for the determination of 7-dehydrocholesterol in human skin surface is described. 7-Dehydrocholesterol, extracted with hexane-ethanol (1:1, v/v) from skin surface using a handy extraction device, is converted into a fluorescent derivative by reaction with 3, 4-dihydro-6, 7-dimethoxy-4-methyl-3-oxo-quinoxaline-2-carbonyl azide. The derivative is separated from biogenic interfering substances on a reversed-phase column (TSK gel ODS-120T) with aqueous 90% (v/v) acetonitrile and detected fluorometrically. The detection limit (S/N=3) is 1.6pmo (0.61ng) cm^-2 of human skin surface [corresponding to 18fmol (6.8pg) in a 10μl injection voumel. The correlation between age and the concentration of 7-dehydrocholesterol in skin surface is also described.
Right : A simple, highly sensitive, high performance liquid chromatographic method with fluorescence detection for the determination of 7-dehydrocholesterol in human skin surface is described. 7-Dehydrocholesterol, extracted with hexane-ethanol (1:1, v/v) from skin surface using a handy extraction device, is converted into a fluorescent derivative by reaction with 3, 4-dihydro-6, 7-dimethoxy-4-methyl-3-oxo-quinoxaline-2-carbonyl azide. The derivative is separated from biogenic interfering substances on a reversed-phase column (TSK gel ODS-120T) with aqueous 90% (v/v) acetonitrile and detected fluorometrically. The detection limit (S/N=3) is 1.6pmo (0.61ng) cm^-2 of human skin surface [corresponding to 18fmol (6.8pg) in a 10μl injection volume]. The correlation between age and the concentration of 7-dehydrocholesterol in skin surface is also described.

Date of correction: June 30, 2006 Reason for correction: - Correction: PDF FILE Details: -

Abstract

A simple, highly sensitive, high performance liquid chromatographic method with fluorescence detection for the determination of 7-dehydrocholesterol in human skin surface is described. 7-Dehydrocholesterol, extracted with hexane-ethanol (1:1, v/v) from skin surface using a handy extraction device, is converted into a fluorescent derivative by reaction with 3, 4-dihydro-6, 7-dimethoxy-4-methyl-3-oxo-quinoxaline-2-carbonyl azide. The derivative is separated from biogenic interfering substances on a reversed-phase column (TSK gel ODS-120T) with aqueous 90% (v/v) acetonitrile and detected fluorometrically. The detection limit (S/N=3) is 1.6pmo (0.61ng) cm^-2 of human skin surface [corresponding to 18fmol (6.8pg) in a 10μl injection volume]. The correlation between age and the concentration of 7-dehydrocholesterol in skin surface is also described.

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