1990 年 6 巻 3 号 p. 341-345
Photoacoustic densitometry by using a fast laser beam with sinusoidal scanning was conducted. The signal distortion was improved by using a sinusoidal scanning system combined with a Bessel high-pass filter. However, small distortions were found to remain in the densitograms, compared with those produced by conventional light scanning. Ovalbumin was two-dimensionally electrophoresed, followed by blotting on a nitrocellulose sheet. The signal intensity of ovalbumin on a nitrocellulose sheet was about 5-times greater than that on polyacrylamide gel. The reproducibility (RSD) of measurements for ovalbumin on four different nitrocellulose sheets was in the 8-15% range. However, the value was less than 5% for measurements of ovalbumin on the same plate. The detection limit of ovalbumin was about 150ng. Ovalbumin, 1.67μg of which was spiked in calf serum, was almost recovered by the method.
