1990 年 6 巻 6 号 p. 797-806
Chemiluminescence analyses of biological constituents using metal-complex catalysts developed in our laboratory are reviewed. This study was carried out in the fields of immunoassay and high performance liquid chromatography, with special reference to the determination of micro amounts of protein. The use of a synthesized metal-complex compound instead of natural substances as a catalyst was proposed in order to make immunoassay inexpensive and reproducible, as well as to make labeling easy. The immunoassay established by the use of a fiber-optic immunosensor could be successfully applied to the determination of micro amounts of protein. The method using the activity lowering of a chemiluminescence catalyst was also established for the development of a new detection system, which was applied to both indirect and direct flow injection analyses of protein. Each chemiluminescence system was utilized for indirect and direct flow injection analyses, respectively. The direct flow injection analysis, in which a protein solution was directly injected into a flow injection system, could be used to determine the protein content more easily, rapidly, and sensitively than the indirect one. Finally, the future prospect is briefly presented regarding the present subject.
