Spectrophotometric Determination of Hydrogen Peroxide by the Formation of Indamine Dye with the Catalyst of Water-Soluble Ironporphyrin

抄録

A water-soluble iron porphyrin, a meso-tetrakis (1-methyl-4-pyridyl)porphinatoiron(III) complex (FeTMPyPX₅; X=OCH₃ or halide), was successfully applied as a catalyst of indamine dye formation, which can be used for the determination of hydrogen peroxide in an acidic media. The dye formation process includes an oxidative coupling of N, N-dimethylaniline and 3-methyl-2-benzothiazolinone hydrazone with hydrogen peroxide. The absorbance of the formed dye was measured at 590nm with a spectrophotometer. The materials were stable and the reaction was specific for hydrogen peroxide without any influence of molecular oxygen at low pH, 1-2. The method showed easy handling in the concentration range of (0.3-3.7)×10^-5mol dm^-3 with a correlation factor: 0.999₈ and RSD=1.7₄%. This method can be used for the determination of hydrogen peroxide generated through enzymatic processes.