抄録
A simple, rapid chemiluminescent photographic method was developed for the assay of hydrogen peroxide or glucose using a camera luminometer. Some chemiluminogenic reagents were tested regarding their ability by using a visual method. 4, 4′-Oxalyl-bis[(trifluoromethylsulfonyl)imino]trimethylene- bis (4-methylmorpholinium) trifluoromethanesulfonate (MPTQ) was found to be a suitable oxamide for the photographic method. Pyrimido[5, 4-d]pyrimidines, dipyridamole (DP), 2, 4, 6, 8-tetramorpholinopyrimido[5, 4-d]pyrimidine (MP) and 2, 4, 6, 8-tetrathiomorpholinopyrimido [5, 4-d]pyrimidine (TMP) were effective enhancers, as were perylene and Rhodamine B, on the peroxyoxalate chemiluminescent reaction examined. Fifty picomoles of hydrogen peroxide per 200μl on a micro-titer plate well could be detected as a white spot on instant film. Glucose was detected after being enzymatically oxidized to hydrogen peroxide with glucose oxidase. The detection limit of glucose was 100pmol per well using MPTQ and MP as chemiluminogenic reagents. By the proposed method, glucose in 50μl of 100-fold diluted human serum could be detected.
