1991 年 7 巻 5 号 p. 727-731
A highly sensitive, selective and rapid reversed-phase liquid chromatographic method using a column-switching technique has been developed for the simultaneous determination of a new dihydropyridine calcium blocker, methyl (±)-4, 7-dihydro-3- isobutyl-6-methyl-4-(3-nitrophenyl)thieno[2, 3-b]pyridine-5-carboxylate (I) and its pyridine metabolite, methyl 3-isobutyl-6-methyl-4-(3-nitrophenyl)thieno[2, 3-b]pyridine-5-carboxylate (II), in dog plasma. Compounds I and II were determined with electrochemical detection at +0.50V and by ultraviolet detection at 250nm, respectively. The limits of the determination for compounds I and II were 0.2 and 2ng ml-1, respectively, using 1ml of dog plasma. Good liner relationships were found between the peak areas and the plasma concentrations of compounds I and II in the ranges from the lower limit of determination to 200ng ml-1. The method was applied to preliminary pharmacokinetic studies after oral administration of compound I to beagle dogs.
