Abstract
The incorporation of various 14C-labeled compounds into the two-carbon side chain of kasugamycin was studied. Among U-14C-maltose, U-14C-glucose, U-14C-mannose, 1-14C-glycerol, U-14C-pyruvate, 1-14C-acetate, U-14C-myoino sitol, 1-14C-glycine, 2-14C-glycine, U-14C-glyoxylate, U-14C-oxalate and U-14C serine tested, 1-14C-glycine and 2-14C-glycine were most highly incorporated into the side chain of kasugamycin. They were almost exclusively incorporated into two carbons of the side chain. U-14C-Serine was incorporated at the rate 4 times less than 2-14C-glycine. A dilution effect of glyoxylate, oxamate and oxalate on the incorporation of 2-14C-glycine was not observed.
