DIFFERENTIAL INHIBITION OF TRANSCRIPTIONS CATALYZED BY T7-SPECIFIC AND ESCHERICHIA COLI RNA POLYMERASES BY BLEOMYCIN

Abstract

The effect of bleomycin on in vitro T7 DNA transcriptions catalyzed by phage T7-specific and Escherichia coli RNA polymerases was investigated. It was observed that T7 RNA polymerase was more readily inhibited by the antibiotic than E. coli RNA polymerase. The possible mechanism which would explain the differential inhibition of the transcription was discussed.