The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
MUTAGENESIS OF OA-6129 CARBAPENEM-PRODUCING BLOCKED MUTANTS AND THE BIOSYNTHESIS OF CARBAPENEMS
IKUO KOJIMAYASUO FUKAGAWAMITSUYASU OKABETOMOYUKI ISHIKURANORIO SHIBAMOTO
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1988 Volume 41 Issue 7 Pages 899-907

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Abstract

Streptomyces fulvoviridis A933 17M9 1501 is an A933 acylase-defective mutant derived from S. fulvoviridis A933 17M9 and thus produces the OA-6129 group of carbapenems and carbapenams. By further mutation of mutant 1501, 4 types of mutants (OA-6129 A+B1+B2 producers; OA-6129 A+B2 producers; an OA-6129 A producer; non-producers) were obtained. The second type of mutant strains 4N 3607, 5NA 3949-40 and 5NE 252 proved useful for the fermentative production of carbapenem OA-6129 B2. These results of mutagenesis demonstrated that the sequence of carbapenem bioconversion in the horizontal route was hydroxylation at C-8→HSomerization at C-6→sulfation at C-8 hydroxyl.

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© Japan Antibiotics Research Association
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