BIOCHEMICAL STUDIES ON 2-DEOXY-SCYLLO-INOSOSE, AN EARLY INTERMEDIATE IN THE BIOSYNTHESIS OF 2-DEOXYSTREPTAMINE II. QUANTITATIVE ANALYSIS OF 2-DEOXY-<i>SCYLLO</i>-INOSOSE

NORIAKI YAMAUCHI; KATSUMI KAKINUMA

doi:10.7164/antibiotics.45.767

The Journal of Antibiotics

Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820

BIOCHEMICAL STUDIES ON 2-DEOXY-SCYLLO-INOSOSE, AN EARLY INTERMEDIATE IN THE BIOSYNTHESIS OF 2-DEOXYSTREPTAMINE

II. QUANTITATIVE ANALYSIS OF 2-DEOXY-SCYLLO-INOSOSE

NORIAKI YAMAUCHI, KATSUMI KAKINUMA

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1992 Volume 45 Issue 5 Pages 767-773

DOI https://doi.org/10.7164/antibiotics.45.767

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Abstract

A practical quantitative analysis of 2-deoxy-scyllo-inosose by means of GC-MS selected-ion monitoring (SIM) was exploited in order to assay the enzymatic 2-deoxy-scyllo-inosose formation, the first stage of the 2-deoxystreptamine biosynthesis. Mass spectral fragmentations of 2-deoxy-scyllo-inosose tetra-O-trimethylsilyl (TMS) ether was also investigated by the use of [2, 2-²H₂]-2-deoxy-scyllo-inosose.

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