DEPUDECIN: A NOVEL COMPOUND INDUCING THE FLAT PHENOTYPE OF NIH3T3 CELLS DOUBLY TRANSFORMED By ras- AND src-ONCOGENE, PRODUCED BY Alternaria brassicicola

Abstract

A novel compound depudecin inducing the flat phenotype of ras- and src- transformed NIH3T3 cells at a concentration of 1 μg/ml was isolated from the culture broth of Alternaria brassicicola. Based on its spectroscopic characteristics and X-ray crystallographic analysis of its bis-(1S)-(-)-camphanate, the structure of depudecin was determined'to be (2R, 3S, 4S, 5E, lS, 8S, 9R)-2, 9-dihydroxy3, 4;7, 8-diepoxy-undeca-5, 10-diene.