IC101, EXTRACELLULAR MATRIX ANTAGONIST PRODUCED BY Streptomyces sp. MJ202-72F3

PRODUCTION, ISOLATION, STRUCTURE DETERMINATION AND BIOLOGICAL ACTIVITY

Abstract

In our search for inhibitors of cell adhesion to components of extracellular matrix (ECM), fibronectin, laminin and collagen type IV, we succeeded in finding a novel cyclic hexadepsipeptide antibiotic, named IC101, which was isolated from cultured mycelium of Streptomyces albulus MJ202-72F3. It was purified by centrifugal partition chromatography, preparative reverse phase HPLC and Sephadex LH-20 and was obtained as a white powder. 1C 101 strongly inhibited cell adhesion to ECM components, suppressed immune responses in vitro and in vivo, and exhibited antimicrobial activity on Gram-positive bacteria.