Heliquinomycin, a New Inhibitor of DNA Helicase, Produced by Streptomyces sp. MJ929-SF2 I. Taxonomy, Production, Isolation, Physico-chemical Properties and Biological Activities

MAKOTO CHINO; KIYOHIRO NISHIKAWA; MAYA UMEKITA; CHIGUSA HAYASHI; TAKAKO YAMAZAKI; TOSHIO TSUCHIDA; TSUTOMU SAWA; MASA HAMADA; TOMIO TAKEUCHI

doi:10.7164/antibiotics.49.752

Heliquinomycin, a New Inhibitor of DNA Helicase, Produced by Streptomyces sp. MJ929-SF2

I. Taxonomy, Production, Isolation, Physico-chemical Properties and Biological Activities

MAKOTO CHINO, KIYOHIRO NISHIKAWA, MAYA UMEKITA, CHIGUSA HAYASHI, TAKAKO YAMAZAKI, TOSHIO TSUCHIDA, TSUTOMU SAWA, MASA HAMADA, TOMIO TAKEUCHI

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JOURNAL FREE ACCESS

1996 Volume 49 Issue 8 Pages 752-757

DOI https://doi.org/10.7164/antibiotics.49.752

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Abstract

Heliquinomycin was isolated as a part of a program designed to find inhibitors of DNA helicase from microbial sources. It was purified from the culture broth of Streptomyces sp. MJ929-SF2 by solvent extraction and serial chromatographies of centrifugal partition chromatography, Sephadex LH-20 and Capcell Pak CIS (HPLC). The isolated red powder was analyzed to have the molecular formula of C₃₃H₃₀O₁₇. It inhibited partially purified DNA helicase from HeLa cell in a noncompetitive manner with the inhibition constant (Ki) of 6.8mM.
Heliquinomycin exhibited biological activity against microorganisms including MRS A, and cultured cell lines.

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