ANALYSIS OF LIPOPOLYSACCHARIDE (LPS) FROM IMPERMEABILITYTYPE DRUG RESISTANT PSEUDOMONAS AERUGINOSA USING NEW, HIGHLY-SENSITIVE LPS STAINING METHOD

Abstract

The specific ladder pattern on polyacrylamide gel electropholesis of lipopolysaccharide (LPS) extracted from Pseudomonas aeruginosa was clearly shown by using the complex methods of the PAS staining, re-periodate oxidation and then Ag-staining method. Accordingly, it was concluded that the new method was grately useful for a detail analysis of LPS changes in Gram-negative bacteria. And it was shown by this method that no changes in LPS occurred between the impermeability-type drug resistant P. aeruginosa mediated by R plasmid and a drug susceptible strain. The absence of changes indicated that the LPS of P. aeruginosa K-Ps102 had no role inthe mechanism of the high drug resistance.