Original articles
Studies on a New Anti-Tumor Antibiotic, Ayamycin. II On the Isolation and Physico-Chemical Properties of Ayamycin A2
1960 年 13 巻 5 号 p. 321-326
詳細
1960 年 13 巻 5 号 p. 321-326
In the previous paper1) of this series, the procedure of isolation of antibiotics ayamycins A and B from the cultured liquid of Streptomyces No. O-80 and their biological properties were presented. At that time, the crude powder was found to exhibit an antitumor effect and anti-bacterial effect. While further fractionation showed that ayamycin A complex was responsible for the major part of the anti-tumor activity in the cultured liquid.
During the course of screening anti-tumor antibiotics in our laboratory, it has been found that many exhibit an anti-tumor effect in the so-called actinomycin-type antibiotics.2) Ayamycin was also included in actinomycin-type antibiotics.3) However, it was of interest to note that this compound differed considerably from the known actinomycin-type antibiotics, in that the in vitro anti-tumor effect was stronger than the antibacterial effect, and that it contained a quinone structure.
In subjecting the ayamycin A complex to a modified Vining’s paper chromatography procedure4) for isolation of actinomycin components, it was found that several components existed, and among these, A2 was obtained as a yellow needle crystal. This A2 crystal was responsible for the major part of the anti-tumor activity in A complex.
In this paper, further details on isolation and purification of ayamycin A complex and physico-chemical characteristics of the crystal obtained from A2 fraction are presented.
