抄録
Minute changes in the liver cells in several stages of the development of cancer in rat's liver after administration of butter-yellow were studied.
1. The animals' weight increased slower. Macroscopic changes appeared in 7 weeks after the beginning of butter-yellow administration, when in total 250mg butter-yellow were given.
2. Under the light microscope, two different features were observed, i. e. predominant multiplication of connective tissue and predominant proliferation of bile duct epithelium. In these two types of tissue, characteristic cells with specific structure appeared. In other liver cells occurred also function changes concerning mitochondria. As common findings for these two types of tissue, there appeared cell-islands and dwarf cells.
3. Where there was a predominance of connective tissue, swollen cells appeared as specific feature after about 3 weeks butter-yellow administration. These cells contained liquid within the cytoplasm, and the mitochondria were fine rods. These cells contained no lipoid and glycogen and showed no morphological changes following an artificial diet. These cells came out first in the peripheral region of the lobule, later in the central area. The mitochondria of these cells became finer and finer and less stainable, and after 15 weeks butter-yellow administration died many of such cells. This type of tissue was assumed to be altering from liver cirrhosis to hepatoma.
4. In the tissue-type in which bile duct epithelium proliferated, lipoid accumulated in the liver cells. The cells should be called therefore inactived cells. In this cells mitochondria became finer and showed no changes after an artificial diet. These cells increased gradually with a proliferation of bile duct epithelium, till they revealed a cholangioma-like picture.
5. The GOLGI's apparatus in liver cells arrayed generally along the cell equator in a form of twist rope or spots and gathered to bile capillaries following an artificial diet. After a long time butter-yellow administration, however, became the apparatus less regular arranged and gathered to bile capillaries slower than by control animal after an diet. In the specific cells, on the other hand, the arrangement of the GOLGI's apparatus was much slender and dispersed, its changes after an diet were not clear to see.
6. Electron microscopic findings of the liver cells were not so distinkt as under the light microscope. Yet were observed irregularity of nucleus, swelling of mitochondria, shortening and disorderly arranging of mitochondrial cristae, decrease of the density of the mitochondria, and also decrease of endoplasmic reticulum.
