A Relation Between Nuclear Size and Composition of its Suspending Media

Abstract

With respect to the relation between nuclear size and chemical composition of its suspending medium, diameters of the nuclei isolated by the technique of sucrose or glycerol were measured in sucrose or glycerol solution in addition with various concentrations of several cations. Obtained results were summarized as follows.
Nuclear sizes were decreased with addition of cations, and on the control of nuclear size, bivalent cations were further effective than univalent. The nuclear size depended only on the concentration of cations, but did not on that of sucrose. Even if the sucrose was substituted with glycerol, similar results were obtained.
These facts throw a question whether a concept of ‘isotony of cell’ is equally fitted for isolated nuclei.