Saliva Splits Histamine (Productin)

I. On Saliva from Human Parotis

Abstract

When comparing the absorption theory of saliva (OGATA) and the Productin theory (FUJIE), the authors were able to find many points to be considered concerning the problem where the saliva can split Productin (histamine) in the blood. To determine these considerations, the authors have performed the following experiments.
1. 1cc of 0.6% histamine solution (6mg of histamine) was mixed with 1cc of aqua dest., then 1cc of the mixture (3mg of histamine) was injected subcutaneously into starved rats (HW).
2. Saliva was gathered from the human parotis, aseptically, according to UMEMOTC-KAKUDO's method. 1cc of saliva was mixed with 1cc of histamine solution, and the mixture was put into an incubator at a temperature of 37°C for 10min 30min., 60min., 3hrs, 6hrs., 24hrs. and 48hrs., then 1cc of the mixture (3mg histamine) was injected subcutaneously into starved rats (HP-10′, HP-6^hrs etc.).
3. 1cc of histamine solution and 2cc of saliva were mixed, and after incubating it at 37°C for 10min., 1cc of the mixture (2mg of histamine) was injected (H2P-10′).
4. Saliva was heated for 30min. at 60°C and 90°C, then was mixed with histamine solution as in (2), 10min, later, 1cc of the mixture was injected (60°C……HP′-10′, 90°C……HP″-10′).
5. Sesame oil (37°C, 1cc) Was instilled orally into the stomach of starved rats (sesame oil).
6. Saliva from human parotis (1cc) was injected subcutaneously, then sesame oil was instilled as in (5) (P injection-sesame oil).
20, 40 and 60min. after the subcutaneous injection, and 30, 60min., 1.5 and 2hrs. after instilling sesame oil, the authors have analysed, quantitatively, histamine in the blood by using NAKAMURA's method and at the same time, observed the functional movement of the minute structure in the peptic and pancreatic cells.
The results obtained here show that after subcutaneous injection of the mixed solution of histamine and saliva the increase of histamine in the blood is slight. The longer the time has passed since the mixing the more marked was the increase of histamine in the blood. When the mixed solution, where saliva was heated to 60°C and 90°C before the mixing, was used, the increase of histamine in the blood was similar to the case when histamine alone was injected (Graph. 1). Here the authors have come to believe that saliva has an action to split histamine enzymatically.
This fact appeared in the value concerning the amount of histamine in the blood when sesame oil was instilled orally into the stomach after subcutaneous injection of saliva (Graph. 2). On the other hand, histamine value in the blood of each experiment can be justified that it is not a inconsistent when observing the minute structure in the functional movement which appeared in the peptic and pancreatic cells.
Therefore, the authors' results ate believed to have given a new significance to the absorption theory of saliva into the blood (OGATA). That is, the saliva, absorbed into blood, has an important meaning in spliting the gastric hormone Productin (histamine), secreted into blood. The action of salive towards histamine is not a chemical reaction but positively enzymatic one.