抄録
Polyhydroxyalkanoate (PHA) and triacylgrycerol (TAG) production by IAR1, a novel toluene-degrading bacterium isolated from soil, was studied. When IAR1 was cultivated on acetate as the sole carbon source, PHA and TAG content attained 12% and 24% of cell dry weight (CDW), respectively. After acetate was exhausted, PHA content decreased and TAG content increased slightly. Thus, IAR1 seemed to synthesize TAG from PHA under carbon-limited condition. When IAR1 was cultivated on toluene as the sole carbon source, PHA content reached 9.3% of CDW and became constant even though toluene was supplied. On the other hand, TAG content increased throughout cultivation and reached 24% of CDW. PHA accumulated by IAR1 was a copolymer of 3-hydroxybutylate (3HB) and 3-hydroxyvalerate (3HV) (PHBV). TAG synthesis by IAR1 was inhibited by adding cerulenin and PHBV content attained 30 % of CDW. Cerulenin addition also affected PHBV composition.
