抄録
A microfluidic device for cell-culture systems has been studied. Microfluidic devices fabricated using polydimethylsiloxane (PDMS) were developed for a culture of plant cells. Cultured strawberry cells, which produce red pigments, anthocyanins, were introduced into a cultivation chamber in the microfluidic device. These devices composed of cultivation chambers, weir structures to retain cultured plant cells in them, several inlets and outlets, and connecting micro-channels. The conditioned medium (CM) combined fresh medium and used-medium was used to facilitate the cell growth at low cell density. In this micro-scale culture system, strawberry cells were successfully proliferated for more than 5 days. Moreover, the cell growth and pigment productivity using CM were compared with those using normal fresh medium.
