Development and Characterization of EST-SSR Markers for Tephroseris furusei (Kitam.) B. Nord. (Asteraceae) and Cross-species Amplification

抄録

Twelve polymorphic EST-SSR markers were developed and characterized for Tephroseris furusei (Kitam.) B. Nord., an endangered species endemic to limestone habitats in central Japan, and related spe- cies. In the two T. furusei populations, the total numbers of alleles for each locus ranged from 5 to 16 (average = 8.75). The observed (H_O) and expected (H_E) heterozygosities ranged from 0.33 to 0.76 (average = 0.58) and 0.50 to 0.89 (average = 0.75), respectively. The inbreeding coefficient (fixation index, F_IS) ranged from 0.14 to 0.50 (average = 0.24) and five loci deviated significantly from Hardy–Weinberg equi- librium. The versatility of the markers was evaluated for three congenerics: T. flammea (Turcz. ex DC.) Holub subsp. flammea, T. integrifolia (L.) Holub subsp. kirilowii (Turcz. ex DC.), and T. pierotii (Miq.) Holub. All of the EST-SSR markers were transferrable among them.